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bsl 2 checklist

BSL2 See agents that require enhanced precautions BSL-2 BSL3. Dispose of used gloves with other contaminated laboratory waste.


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When experiments are in progress.

. Are workspaces un-crowded. You can use this checklist to help prepare for your biosafety lab inspection and to easily see the requirements for BSL-2 laboratories. Examples of agents typically worked with in a BSL-2 include equine encephalitis viruses and HIV as well as Staphylococcus aureus staph infections.

Work surfaces are decontaminated at least once a day and afte r any spill of infectious or hazardous age nts. Required for all work. BMBLC4-c In addition BSL-2 laboratory workers should.

The following are procedures are used with BSL-2 containment requirements. Persons wash their hands after they handle viable materials and animals after removing gloves and before leaving the laboratory. Eating drinking smoking handling contact lenses and applying cosmetics are not permitted in the work areas.

Import Permit Program ABSL-2 Inspection Checklist Page 4 of 11. Biosafety Level 2 Section 1. Are benches and shelves never overloaded.

Smoking chewing gum handling contacts applying cosmetics is. Microsoft Word - BSL-2 Checklistdoc Version. Class II Biological safety cabinet with annual certification Not required.

The PI has a documented training program for all persons working in the laboratory. Personnel must have specific training in the procedures conducted in the BSL-2 cleanroom or laboratory. Are chairs appropriate for laboratory environment.

Standard Microbiological Practices Access Hand washing Food Eye protection Pipetting Sharps Splashes Aerosols Decontamination. 112021 If a lab has windows that open they are fitted with fly screens or the lab has a policy not to open windows. Access to the laboratory is limited or restricted at the discretion of the PI.

Mechanical pipetting devices are available and used for all pipetting. Biosafety Level OneTwo BSL-12 Inspection Checklist ASUs research and teaching laboratories that work with biohazards are required to have annual inspections by EHS Biosafety and Biosecurity professionals. 11618 Laboratory Assistance Visit Checklist BSL2 laboratories A procedure-based approach comprising the practices personal protective equipment facilities and implementation of your laboratorys Standard Operating Procedures SOPs must be taken when evaluating safety and compliance.

Potentially infectious material is placed in a durable leakproof container during collection handling processing storage or transport within a facility. Revision 2 421 BSL-2 Commissioning Checklist Page 2 of 2 contaminated materials if they are removed from the laboratory for autoclaving. BMBLC4-c Hand washing protocols must be rigorously followed.

Revised May 2021 Yes No NA Response Reference Statement. BSL2 This biosafety level covers laboratories that work with agents associated with human diseases ie. BioSafety Level 2 Checklist Page 8of 11 General Safety Is lab space being utilized safely.

Laboratory personnel should use this checklist to identify safety and regulatory deficiencies and address. A15-d Broken glassware is not. This checklist will be used during the annual inspections.

Microbiological practices before working with BSL2 agents. Gloves are worn when handling infected animals and when skin contact with infectious materials is unavoidable. Bitte geben Sie bei den einzelnen Fragen an ob und wenn ja wie häufig sie die einzelnen.

BSL-2 Inspection Checklist - Rev. O Do chairs have a 5-star base. Experiment areas of lesser biohazard potential are carefully demarcated.

Eating drinking handling contact lenses and applying cosmetics is forbidden in the laboratory. O Are chairs non-porous and cleanable. 072321 Laboratory Assistance Visit Checklist BSL2 laboratories A procedure-based approach comprising the practices personal protective equipment facilities and implementation of your laboratorys Standard Operating Procedures SOPs must be taken when evaluating safety and compliance.

They are based on the recommendation of the Biosafety in Microbiological and Biomedical Laboratories BMBL 5th Edition 2007. Microsoft Word - BSL-2 Checklistdoc Version. Mouth pipetting is prohibited and only mechanical pipetting devices are used.

Required for all aerosol generating processes Required for all work. Items zur Erfassung des Verhaltens Zum Abschluss möchten wir Ihnen noch einige Fragen zu einzelnen Verhaltensweisen stellen. Personnel are trained to handle pathogenic agents and are supervised by those with advanced trainingundefined Biosafety cabinets or other physical containment for all procedures that can generate infectious aerosols or splashes.

You can review your biosafety inspection results and respond to any findings within the online EHSA system for inspections taking place in February 2020 and beyond. Lab equipment is routinely decontaminated as well as after spills splashes or other potential. Yes no NA No food or drinks consumed or stored in the lab.

All personnel wash their hands after they handle viable materials and animals after removing gloves and before leaving the laboratory. Standard Microbiological Practices 1. Biosafety in Microbiological and Biomedical Laboratories CDC.

BSL-2 inspection checklist 15754KB pdf. All contaminated liquid or solid. Personal protective equipment is used based on risk assessment determinations.

BMBLC5 Eye face and respiratory protection should be used in rooms containing infected animals as determined by the risk assessment. By Nimonik Biosafety Level 2 Checklist BSL-2 Loading. O Are chairs undamaged.

Appendix 11101 Revision 0 BSL-2 Commissioning Checklist Page 2 of 3 0704 laboratory coats are removed before leaving the animal facility. Pathogenic or infections organisms that pose a moderate health hazard. A15-c Non-disposable sharps eg necropsy instruments such as forceps pins reusable scalpels are placed in a hard-walled container for transport to a processing area for decontamination.


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